Screening of Diclofenac for Antibacterial activity against Pathogenic Microorganisms

Diclofenac 2(2,6 dichloranilino) phenylacetic acid is a widely prescribed non-steroidal anti inflammatory drug. It is used to reduce pain and treat inflammation in some disorders like rheumatoid arthritis, osteoarthritis, ankylosing spondilytis, gout, dysmenorrhea, spondylarthritis etc., It acts by inhibiting the action of cycloxygenase enzymes COX-1 and COX-2 which are responsible for the synthesis of prostaglandins. Apart from antiinflammatory property, Diclofenac also has powerful antibacterial potential. This study aims to assess the antibacterial effect of diclofenac against five pathogenic microorganisms Bacillus subtilis, Staphylococcus aureus, Eschereschia coli, Pseudomonas aeruginosa, Salmonella typhi. The Agar well diffusion method was adopted to assess the antibacterial activity. The inhibition of bacterial growth was studied taking test drug in three different concentrations 25μg/ml, 50μg/ml and 100μg/ml. The findings of the study indicated the inhibition of growth of bacteria.